Fig. 2 | Scientific Reports

Fig. 2

From: Advancing in ovo egg sexing through molecular sex identification of chick embryos using LAMP and RPA assays

Fig. 2

Validation of sex identification in 82 chick embryo samples using the SWIM/12S assay. Capillary electrophoresis results for the previously established SWIM/12S assay confirm the accuracy of sex determination across 82 chick embryo samples (A) Superimposed electropherograms of all 82 samples. (B) Electropherograms of male samples, showing amplification of the 12 S amplicon only. (C) Electropherograms of female samples, exhibiting amplification of both 12 S and SWIM amplicons. The 12 S amplicons is represented in orange, while SWIM amplicon is shown in green. In panels B and C, the samples are arranged in increasing order of normalized 12 S peak time rather by reference order.

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