Fig. 3

Rapid sex identification of chick embryos using the AC18 LAMP assay. (A) Real-time fluorescence curves of AC18 LAMP assay for the 82 chick embryo samples and the positive control (Plasmid containing AC18 fragment). (B) Ct values (initiation of the exponential amplification) plotted against sample reference names. (C) Ct values as a function of extracted DNA concentration.