Fig. 6

Alpha fold modeling of WRKY25, WRKY18 and the two chimeric proteins. (A) WRKY25 protein model illustrating the putative NOS bridge formed between Lys and Cys residues at the N-terminal region and at the C-terminal region. (B) WRKY18 protein model showing the presence of a Cys residue but the absence of the Lys residue is necessary to form the NOS bridge. (C) W18N-W25C chimeric protein model, depicting the putative NOS bridge in the C-terminal region. (D) W25N-W18C chimeric protein model, illustrating the putative NOS bridge in the N-terminal region. In all models, Lys residues are shown in pink, and Cys residues are shown in red, and the conserved WRKY motif (WRKYGQ) is depicted in mint. Grey arrows indicate closeups of the respective regions in the DBDs with the possible NOS bridges between Lys and Cys.