Fig. 4
From: Magnetic nanoparticles influence the biological function of mesenchymal stem cells
MNPs alters proteomic profiles of MSC. (A) Volcano plot showing the proteins that were upregulated and downregulated in the MSC-MNPs group compared to the MSC group. DIA proteomics revealed 93 upregulated DEPs and 144 downregulated DEPs in the MSC-MNPs group compared with the MSC group (FDR < 0.05, |FoldChange|>1.5), including polarization and axon guidance related proteins, pro-inflammatory related proteins and anti-inflammatory related proteins. (B) Heatmap showing that MNPs uptake by MSC promoted the expression of cytokine-related proteins. (C, D) Western blot analysis verified the upregulation of SLIT2, HGFAC, CD55, APOA1 and MDK and the downregulation of TRAF3 and CHI3L1 in the MSC-MNPs group compared with those in the MSC group. The first three lanes correspond to three samples from the MSC group, while the last three lanes correspond to three samples from the MSC + MNPs group, as indicated in the figure. (E) KEGG pathway enrichment analysis revealed that these DEPs were clustered in the top 15 signaling pathways related to Complement and coagulation cascades and Axon guidance pathway. *P < 0.05, **P < 0.01, ***P < 0.001, triplicate experiments in each group, Student’s t-test.
