Fig. 25

Pseudotime Trajectory Analysis in RAP Patients. Pseudotime and entropy metrics reveal dynamic state transitions among immune cell types during sepsis in a rapid recovery patient (RAP). (A) tSNE plot of immune cells from a rapid recovery patient (RAP), annotated by major cell types using the majority-votingmethod. (B,C) Cells originate from classical monocytes (Classical Mono), progress through DC2 and plasmacytoid dendritic cells (pDC), then differentiate into megakaryocytes/platelets, plasmablasts, and non-classical monocytes, before transitioning into T-cell populations and ultimately CD16⁺ NK cells. (D–M) Ten subpanels depict the probability distributions for each cell’s commitment to the ten terminal states, confirming the logical consistency between the designated root population and terminal differentiation outcomes. Pseudotime: inferred progression order; Entropy: measure of cell-state diversity.