Fig. 26
Dynamic Differentiation Probabilities in RAP Patient Along Pseudotime. Fate probability represents the predicted likelihood of each cell to differentiate into a given terminal subcluster, with higher values indicating stronger commitment. “Selected Cells” highlights the target lineage, contrasted against “Other Cells” to emphasize lineage specificity. Panels A–M depict how differentiation probabilities evolve over pseudotime: during the early phase (D,F,H), cells primarily commit to monocytes and megakaryocytes/platelets, reflecting innate immune dominance; in the mid phase (A,E), commitment shifts toward Tem/effector helper T cells and CD16⁺ NK cells, indicating adaptive immune engagement; and in the late phase (B,J), Tem/Trm cytotoxic T cells and Tcm/naïve helper T cells predominate, signifying the transition to regulatory and reparative states. Notably, CCL5 expression remains enriched in megakaryocytes/platelets, Tem/Trm cytotoxic T cells, and CD16⁺ NK cells throughout pseudotime, suggesting a sustained chemokine-mediated bridge between inflammatory activation and immune regulation.
