Fig. 12 | Scientific Reports

Fig. 12

From: Biophysical characterization and solution structure of the cannulae-forming protein CanA from the hyperthermophilic archaeon Pyrodictium abyssi

Fig. 12

Ca2+-binding and Mg2+-binding to K1-CanA. [1H-15N]-SOFAST-HMQC of 0.12 mM 15N-enriched K1-CanA in 50 mM TRIS/HCl, pH = 6.6, 80 mM NaCl, 10% D2O, 0.4 mM DSS. Temperature 323 K, 1H-resonance frequency 800 MHz. (Blue) Reference spectrum; (red) CaCl2 added to an end concentration of 6.4 mM; (green) MgCl2 added to an end concentration of 6.4 mM.

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