Fig. 15
Ca2+-binding to K1-CanA and CanA. (Left) K1-CanA, (right) CanA. The changes Δδcomb of the amide groups are plotted as a function of the total CaCl2 concentration. (
) D42 (24 mM; 50 mM), (
) D57 (0.74 mM; 0.88 mM), (
) E97 (0.74 mM; 1.1 mM), (
) D114 (0.58 mM; 1.6 mM), (
) E116 (0.73 mM; 0.94 mM), (
) D140 (4.5 mM; 7 mM), (
) E164 (7 mM; 28 mM), (
) D169 (18 mM; 12 mM), (
) K99 (0.69 mM; 0.88 mM), (
) K115 (0.74 mM; 0.95 mM). Values in brackets are dissociation constants KD calculated for each amino acid individually for K1-CanA and CanA, respectively.
