Fig. 16
Mg2+-binding to K1-CanA and CanA. (Left) K1-CanA, (right) CanA. The changes Δδcomb of the amide groups are plotted as a function of the total MgCl2 concentration. (
) D24 (10.3 mM; 12 mM), (
) D42 (12 mM; 40 mM), (
) D43 (15.4 mM; 18 mM), (
) D57 (3.3 mM; 6.8 mM), (
) E97 (3.6 mM; -), (
) D114 (4.6 mM; 9 mM), (
) E116 (3.36 mM; 7.7 mM), (
) D140 (9.3 mM; 23 mM), (
) E164 (8.0 mM; 6.4 mM), (
) D169 (11.1 mM; 1), (
) K59 (4.1 mM; -), (
) K99 (4.0 mM; 5.3 mM), (
) K115 (3.7 mM; 7.7 mM), (
) K157 (7.7 mM; 15 mM). Values in brackets are dissociation constants KD calculated for each amino acid individually for K1-CanA and CanA, respectively.
