Fig. 17

Structural differences between free CanA in solution and CanA as part of the cannulae. (Top left) NMR structure of K1-CanA (this paper), (top right) EM-structure of CanA (PDB accession # 7UII). Arrows show regions with highly significant structural differences. (Bottom) Sequence-dependent local RMSDs of the two structures. Values above the reduced standard deviation to zero σ0 (solid line) are considered significant, and those above 2 σ0 (broken line) as highly significant. β-pleated sheets are colored in blue, α-helices in orange. In addition, the secondary structure elements are depicted schematically. Structures were represented by MolMol15.