Fig. 7
Time course of polymerization of CanA. (Left) 1H-spectra of CanA after starting the polymerization at 298 K by the addition of MgCl2 and CaCl2 up to end concentrations of 20 mM. The sample contained 0.97 mM CanA in 50 mM Tris–HCl, 50 mM NaCl, pH 7.5, 10% D2O, 0.4 mM DSS. The top spectrum was recorded directly after the addition of the divalent ions (dark blue), after 1 h 12 min (red), after 2 h 24 min (green), after 3 h 36 min (magenta), after 4 h 48 min (orange), and after 5 h 57 min (light blue). (Right) Fit of the signal intensity in the ppm range (-0.6, 0.1) due to polymerization by a single (blue) or a double (red) exponential decay. The obtained rate constants for a fit with a single exponential kpol, and a double exponential kpol,1 and kpol,2 are 0.14 ms-1, 0.19 ms-1, and 0.03 ms-1, respectively.
