Fig. 15

Global structural instability of the 5XTD–Metformin complex over 100 ns. (A) RMSD shows large deviations (avg. 3.56 ± 0.82 nm) with a spike to ~ 4.5 nm at 1.16 ns and sustained fluctuations, indicating conformational lability. (B) Radius of gyration increases (mean 4.65 ± 0.53 nm), with unfolding events at 1.16 and 1.6 ns, reflecting loss of compactness. (C) SASA (avg. 513.4 ± 8.2 nm2) shows transient rises, suggesting hydrophobic exposure and tertiary destabilization, particularly in NDUFV1.