Table 2. iPBS-retrotransposons primer names, sequence, the melting temperature (Tm), CG content (%) and annealing temperature used in this study.
iPBS Primers | Sequence (5’-3’) | Tm (°C) | CG1 (%) | Optimal Annealing Ta (°C) |
|---|---|---|---|---|
2078 | GCGGAGTCGCCA | 54.2 | 75.0 | 62.8 |
2079 | AGGTGGGCGCCA | 56.6 | 75.0 | 65.2 |
2221 | ACCTAGCTCACGATGCCA | 58.0 | 55.6 | 56.9 |
2276 | ACCTCTGATACCA | 42.7 | 46.2 | 51.7 |
2298 | AGAAGAGCTCTGATACCA | 51.6 | 44.4 | 60.0 |
2377 | ACGAAGGGACCA | 47.2 | 58.3 | 53.0 |
2380 | CAACCTGATCCA | 41.4 | 50.0 | 50.5 |
2381 | GTCCATCTTCCA | 40.9 | 50.0 | 50.0 |
2384 | GTAATGGGTCCA | 40.9 | 50.0 | 50.0 |
2391 | ATCTGTCAGCCA | 43.6 | 50.0 | 52.6 |
