Table 4 Mating frequency treatment setup for A. japonicus males at different time intervals.

From: Novel insights into courtship and mating behavior of Anastatus japonicus enhance pest control and mass-rearing efficiency

Mating status of male < 6 h*

Introduction of unexperienced virgin female at different time intervals

6 h

12 h

24 h

48 h

72 h

Virgin

Virgin

Virgin

Virgin

Virgin

Virgin

Honey fed mated

Virgin

Virgin

Virgin

Virgin

Virgin

Starved mated

Virgin

Virgin

Virgin

Virgin

Virgin

  1. *Males were divided in three categories based on the age; younger wasps (0 h–12 h old); mid age wasps (48 h); old wasps (72 h).