Fig. 2
(A) C57BL/6J ENDO mice showed significantly reduced burrowing activity, heightened mechanical sensitivity (Von Frey), and altered Open Field Test (OFT) parameters (decreased central zone duration/entries, increased peripheral zone time) compared to controls, indicative of pain-related behavior. (B) BALB/c ENDO mice also exhibited significant impairment in burrowing, increased Von Frey sensitivity, and reduced central zone activity in OFT, suggesting persistent pain or anxiety-like behavior. (C) Swiss albino ENDO mice displayed diminished burrowing, heightened mechanical sensitivity, and decreased central zone activity in OFT, consistent with pain-related behavioral modifications observed in other strains. While no significant strain-specific differences were observed, BALB/c and C57BL/6J mice showed a tendency towards a more pronounced burrowing deficit. (n = 6 per control group, n = 14 per ENDO group for C57BL/6J and Swiss albino, n = 12 per ENDO group for BALB/c; ***P < 0.001, ****P < 0.0001). Trajectory plots for C57BL/6J (G), BALB/c (H), and Swiss albino (I) ENDO and control mice in the Open Field Test, visually confirming diminished overall movement in ENDO mice.(D, E, F) Significantly heightened mechanical hypersensitivity in ENDO mice of all strains compared to their respective control groups. (J, K, L) Statistically significant decrease in the number of entries into the central and peripheral zones, a shorter duration of time spent in the central area, and increased time spent in the peripheral zones in the ENDO groups compared to the controls.
