Table 1 A list of cell lines/bacterial strains, their origin and maintenance/growth conditions used within this study.
From: Antibiotic carry over is a confounding factor for cell-based antimicrobial research applications
Human immortalised cell lines | Origin | Routine growth medium/conditions | Reference |
|---|---|---|---|
10PCAh | Immortalised human oral mucosal lamina propria-progenitor cells | DMEM, 10% FBS, 1% L glutamine, 1% Antibiotic/Antimycotic 37 °C + 5% CO2 | |
NHh | Immortalised human fibroblasts from healthy leg tissue (patient H) | ||
NIh | Immortalised human fibroblasts from healthy leg tissue (patient I) | ||
NKh | Immortalised human fibroblasts from healthy leg tissue (patient K) | ||
WHh | Immortalised human fibroblasts from chronic venous wound tissue (patient H) | ||
WIh | Immortalised human fibroblasts from chronic venous wound tissue (patient I) | ||
WKh | Immortalised human fibroblasts from chronic venous wound tissue (patient K) | ||
HaCaT | Spontaneously transformed human keratinocyte cell line | ||
DU145 | Prostate carcinoma cell line | ||
Bacterial isolates/strains | Origin (penicillin resistance status) | Bacterial growth medium/conditions | Reference |
S. aureus NCTC 6571 | Lab strain susceptible to penicillin | TSA, TSB, MHB, BHI 35 °C/37°C ± 5% CO2 | ~In house |
S. aureus 1061 A | Clinical strain resistant to penicillin | ||
S. aureus 1004 A | Clinical strain resistant to penicillin | ||
S. aureus 1038B | Clinical strain susceptible to penicillin | ||
S. aureus NCTC 4137 | Lab strain susceptible to penicillin | ~In house | |
S. aureus NCTC 7791 | Lab strain susceptible to penicillin | ~In house | |
S. aureus EMRSA-15 | Clinical strain resistant to penicillin | ~In house | |
S. epidermidis 1064 A | Clinical strain resistant to penicillin | ||
S. epidermidis ATCC 12,228 | Lab strain susceptible to penicillin | ~In house | |
S. warneri NCTC 5955 | Lab strain susceptible to penicillin | ~In house |
