Table 4 Comparative biochemical and cytotoxic properties of L-glutaminase enzymes from various microbial sources.

From: Revolutionizing cancer treatment with Halomonas Aquamarina L-Glutaminase: insights from in vitro and computational studies

Parameter

H. aquamarina HBIM1

(This study)

H. meridiana 32

S. avermitilis 46

B. subtilis 47

Enzyme yield (U/ml)

39.82

32.5

28.4

25.6

Specific activity (U/mg)

748.35

523.6

480.1

410.2

Optimal pH

8.0

7.5

7.0

7.0

Optimal temperature (°C)

50

45

40

37

Km (mM⁻¹)

0.198

0.244

0.285

0.315

IC50

(HepG2, µg/ml)

33.98

48.9

52.3

60.2

IC50

(WI-38, µg/ml)

93.43

Not reported

Not reported

Not reported

  1. Data highlight the superior performance of Halomonas Aquamarina HBIM1 in terms of production yield, specific activity, enzyme stability, substrate affinity, and selective anticancer activity.
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