Fig. 3

Construction of WGCNA co-expression network. (A) Sample clustering dendrogram with tree leaves corresponding to individual samples. (B) The screen of the best soft thresholds. Nine was considered the best soft threshold. (C) The merging of similar modules. (D) Correlations between different modules and clinical traits. Red represents a positive correlation, and blue represents a negative correlation. (E) Collinear heat map of module feature genes. Red color indicates a high correlation, blue color indicates opposite results. (F) Clustering dendrogram of module feature genes. (G,H) The significance of genes related to ALI in the brown and cyan module (a dot represents the genes in the module). (I) A Venn diagram illustrated the overlap between diagnostic markers identified through DEGs and WGCNA. DEGs, Differentially Expressed Genes. ALI, Acute Lung Injury. WGCNA, Weighted Gene Co-Expression Network Analysis.