Fig. 6
Key genes expression analysis in the single-cell transcriptome dataset GSE235367. (A,B) UMAP visualization of all cells in 23 clusters. Each dot represents a cell whose color is coded according to the different clusters. Clusters are named by the most specific and highly cross-gene interpretation. (C) The top5 expressed marker genes for each cell type. (D) Histogram indicated the proportion of cells in the lung tissue for both analyzed samples. (E) The scatter plot shows the top 5 and bottom 5 expression marker genes for each cell type. (F) GO enrichment analysis results in ALI and control samples. (G) Violin plot illustrates the expression levels of four core genes (Cebpd, Hspa12b, Pim1 and Il1r2) in ALI and control samples. (H) Violin plot illustrates the expression levels of four core genes (Cebpd, Hspa12b, Pim1 and Il1r2) in each cell type. (I) The UMAP plots illustrates the expression distribution of Il1r2. p-values were calculated as mean ± SD, P < 0.05 were considered statistically significant differences. *P < 0.05; **P < 0.01; ***P < 0.005. Two-tailed unpaired Student’s t - test for two groups or one-way ANOVA for three groups or more. GO, Gene Ontology. ALI, Acute Lung Injury. UMAP, Uniform Manifold Approximation and Projection.
