Fig. 4
Flow Cytometry Analysis of Different T Cell Phenotypes. (A-C) t-SNE visualization of CD4 + T and CD8 + T cells in immune compartments, categorized by source. (D) Frequencies of PD-1, Tim-3, CD39 and CD103 on CD4 + T cells from PBMC, P, and T samples. (E) Frequencies of PD-1, Tim-3, CD39 and CD103 on CD8 + T cells from PBMC, P, and T samples. (Data are presented as mean ± SD). t-SNE: t-distributed Stochastic Neighbor Embedding.Depending on data normality and variance homogeneity, ANOVA, Welch’s ANOVA, or Kruskal–Wallis test with appropriate post hoc comparisons was used in D and E. *p < 0.05, **p < 0.01, ***p < 0.001.t-SNE, t-distributed stochastic neighbour embedding; PBMC, peripheral blood mononuclear cells; P, peritumoural tissue; T, tumour tissue; CD4, cluster of differentiation 4; CD8, cluster of differentiation 8; PD-1, programmed cell death protein 1; Tim-3, T cell immunoglobulin and mucin-domain containing-3; CD39, ectonucleoside triphosphate diphosphohydrolase-1; CD103, integrin αE.
