Fig. 2 | Scientific Reports

Fig. 2

From: Substrate stiffness regulates triple-negative breast cancer signaling through CXCR4 receptor dynamics

Fig. 2

Enhanced matrix stiffness promotes altered CXCR4 localization. (A) Representative images of SUM149 and Vari068 cells stably expressing CXCR4-BFP cultured on soft (1.5 kPa, left) and stiff (28 kPa, right) matrices under baseline signaling conditions described in methods. Scale bar is 50 μm. (B) Representative line intensity and correlated histograms showing increased cell membrane intensity of CXCR4 in cells cultured on a stiff (28 kPa) matrix of normalized images. (C) Graphs show quantification (n = 10) of the cell membrane of CXCR4 in cells cultured on soft (1.5 kPa) versus stiff (28 kPa) matrices. Similar results were obtained in one additional replicate. *p < 0.05, **p < 0.01.

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