Fig. 3
From: In-frame germline TP53 variant impairs p53 oligomerization and predisposes to cancer
Impaired tetramerization of the TP53-E339_F341del mutant. (A) RPE-TP53-KO and RPE-TP53-KO cells stably transfected with wt-p53, p53-E339G and p53-E339_F341del plasmids were treated with doxycycline and nutlin-3 for 12 h. Cell extracts were cross-linked with glutaraldehyde and separated on SDS-PAGE. Staining for GAPDH protein was used as a loading control. Representative image from three experiments. (B) RPE-TP53-KO and RPE-TP53-KO cells stably transfected with wt-p53 (positive control) or p53-E339_F341del plasmids were treated with doxycycline, seeded on 6-well plates and were grown in the presence of nutlin-3 for 10 days. Upon fixation, the number of colonies was counted and normalized to the RPE-TP53-KO cells, error bars indicate SD, n = 3. Statistical significance was determined by t-test, **P < 0.01.
