Fig. 7
The synergistic inhibition of CTXA combined with cDDP on cell growth of A549 cells. (A) A549 cells were treated with various doses of cDDP. (B) A549 cells were incubated with CTXA (3 µM) and cDDP (12 µM) in combination or individually for 24 h. (C) A549 cells were incubated with CTXA (3 µM) and cDDP (15 µM) in combination or individually for 24 h. (D) A549 cells were incubated with CTXA (3 µM) and cDDP (15 µM) in combination or individually for 1 h, and then expression levels of EGFR pathway were detected by Western blot. (E–G) Quantification of the p-EGFR, p-Erk and p-AKT levels versus their relative total protein levels was analyzed. Data represent the average of three independent experiments (means ± SEM). ##p < 0.01, ###p < 0.001 versus the control; ***p < 0.001 versus EGF.
