Fig. 3
From: Glucocorticoids induce femoral head necrosis in rats through the HIF-1α/VEGF signaling pathway
Assessment of the transfection efficiency of HIF-1α-OE and its effect on the HIF-1α/VEGF signaling pathway, as well as the osteogenic differentiation in DEX-induced MC3T3-E1 cells. (A) HIF-1α protein levels in HIF-1α-OE cells were measured via WB. (B) Quantitative assessment of HIF-1α levels. (C) RT-PCR analysis of HIF-1α mRNA levels. (D) WB analysis of VEGF, Runx2, HIF-1α, ALP, and OPN protein levels in DEX-treated and HIF-1α overexpressing MC3T3-E1 cells, respectively. (E) Statistical analysis of protein expression of HIF-1α, VEGF pathway in DEX-treated and HIF-1α overexpressing MC3T3-E1 cells, respectively. (F) Statistical analysis of the protein expression of osteogenic markers, Runx2, ALP, and OPN, in DEX-treated and HIF-1α overexpressing MC3T3-E1 cells, respectively. “ns” stands for “no significant difference,” ***p < 0.001, **p < 0.01, *p < 0.05, n = 3.
