Fig. 4
From: Glucocorticoids induce femoral head necrosis in rats through the HIF-1α/VEGF signaling pathway
Effect of HIF-1α-OE on DEX-induced osteogenic staining of MC3T3-E1 cells in vitro. (A) ALP activity was measured via ALP staining (Scale bar = 100 μm). (B) Calcified nodules were observed via ARS (Scale bar = 100 μm). (C) Quantitative assessment of ALP staining intensity. (D) Quantitative assessment of ARS intensity. “ns” stands for “no significant difference,” ***p < 0.001, **p < 0.01, *p < 0.05, n = 3.
