Fig. 5
Assessment of potential RF-EMF-induced adaptive response in XP6BE fibroblasts. Cells were exposed to sham or 5G-modulated 3.5 GHz RF-EMF at 0.08 or 4 W/kg for 20 h, followed by a 3 h resting phase. Then, increasing challenging dose (CD) concentrations of As₂O₃ were applied for 30 min before BRET measurement, as illustrated in Fig. 1D. (A and B) Dose response curves of As2O3 obtained with XP6BE fibroblast cells expressing the cytoplasmic ROBINy and being exposed to RF-EMF at SAR of 0.08 W/kg (A) or 4W/kg (B). (C and D) Dose response curves of As2O3 obtained with XP6BE fibroblast cells expressing the mitochondrial ROBINy BRET probe and being exposed to RF EMF at SAR of 0.08 W/kg (C) or 4 W/kg (D). (E–G) Box plots showing basal BRET (E), pEC50 of As2O3 (F), and maximal efficacy of As2O3 (G) across the different SAR conditions. No adaptive response was observed following RF-EMF exposure, in contrast to As₂O₃-induced adaptation (Fig. 4). Statistical analysis was conducted using the Kruskal–Wallis Test. n = 8–9 depending on experimental conditions. n.s.: not significant.
