Fig. 2

Flow cytometry characterization of gastric cancer stem cells (CSCs). (A) Gating strategy to exclude out-of-focus cells, debris, doublets and cell clumps. (B) Characterization of AGS CSCs, showing high expression of CD44+, CD166+, and EpCAM+. (C) Characterization of KATO III CSCs, which displayed high levels of CD44+, CD133+and EpCAM+.