Fig. 4
Cellular cytotoxicity of DSBCl at concentrations of 1.25, 2.5, 5, 10, 20, 40, 80, and 160 µg/mL in AGS CSCs (A), KATO III CSCs (B), and 293T cells (C) after 24 and 48 h of treatment. Cell viability was assessed via a resazurin assay, where live cells reduce resazurin (blue) to resorufin (pink), whereas dead cells retain blue. The bar graphs show the mean cell viability (%) ± SD, and the dose‒response curves represent the IC50 values. (D) Comparison of IC50 values for 293T, AGS, and KATO III CSCs at each time point. C+: cells treated with 15% DMSO in culture medium (positive cytotoxicity control). C−: cells treated with 0.1% DMSO in culture medium (vehicle control). Statistical significance: *p < 0.05; **p < 0. 01; ***p < 0.001; ns not significant.
