Table 4 Alterations in the intensities of main functional groups in the second derivative FTIR data in 1700–1600 cm−1 region of FTIR spectra representing the alterations in protein secondary structure for ADSCs in control, 1 H post-L and 3 H post-L groups.

From: FTIR spectroscopic characterization reveals short-term macromolecular responses to photobiomodulation in mesenchymal stem cells

Functional Groups

Control

1 H post-L

3 H post-L

Antiparallel β-sheet structure (at 1681 cm−1)

−0.091 ± 0.008

−0.049 ± 0.003**

−0.110 ± 0.011‡‡‡

α-helical structure (at 1659 cm−1)

−0.159 ± 0.015

−0.113 ± 0.007*

−0.202 ± 0.007*, ‡‡‡

Native β-sheet structure (at 1637 cm−1)

−0.121 ± 0.019

−0.115 ± 0.006

−0.128 ± 0.019

Aggregated β-sheet structure (at 1628 cm−1)

−0.117 ± 0.010

−0.137 ± 0.013

−0.095 ± 0.006‡

  1. The data were represented as mean ± standard error of mean. Comparison was performed by the one-way ANOVA test with Tukey’s as a post-hoc test. A p value equal or less than 0.05 was considered as statistically significant (*p < 0.05, **p < 0.01, ***p < 0.001). The degree of significance was denoted by * for the comparison of control with the other groups, by ‡ for the comparison of 1 H post-L group with 3 H post-L group.
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