Fig. 2
mic-miR-221 and siRNA transfection effectively interfered with the expression of Sox11. (A) Western blot analysis showed that Sox11 protein levels are decreased in the Si-Sox11 treatment group but increased in the ICH + mic-miR-221 group. (B) Quantification of protein levels of Sox11 in rats in various groups. (C) Double-immunofluorescence analysis was performed with antibodies for Sox11 and NeuN (neuronal marker, red) and nuclei was fluorescently labeled with DAPI. Arrows point to Sox11-positive neurons. Scale bar = 20 μm. (D) Quantification of the relative fluorescence intensity of Sox11. ICH group compared with the Sham group; ICH + mic-miR-221 group compared with the ICH + Vector group; ICH + Si-Sox11 group compared with the ICH + Si-Control group. ** P < 0.01. All data are expressed as mean ± SD, n = 6.
