Fig. 4

Genetic or chemical Atf6 inactivation downregulated UPR-regulated gene set. (a) Violin plot shows expression levels of 118 UPR-regulated genes in P17 Atf6^−/− OIR retina RNA-Seq transcriptomes. Expression levels are shown as log₂fold change compared to Atf6^+/+ OIR retinas (red violin plot, ***p ≤ 0.001, Two-Tailed Wilcoxon Signed Rank Test, n = 5/group). The complete UPR gene set is shown in Supplemental Table 5. (b) Violin plot shows expression levels of 118 UPR-regulated genes in wild-type P30 retinas treated with Ceapin-A7, 24 h post-injection. The thick dashed line indicates the median log₂(fold change), the thin dotted lines indicate the top and bottom quartiles, and the blue line indicates 1x fold change (purple violin plot, ****p ≤ 0.0001, Two-Tailed Wilcoxon Signed Rank Test, n = 5/group; Supplemental Table 7).