Fig. 8 | Scientific Reports

Fig. 8

From: Flecainide mediated sodium channel blockade enhances blood brain barrier integrity and promotes neuroprotection in neuroinflammation

Fig. 8The alternative text for this image may have been generated using AI.

In vitro flecainide treatment of pMBMECs alters gene expression profile and protein expression of important BBB integrity markers. (A) The graph depicts the ΔΔCt values (normalized to housekeeping gene GAPDH and relative to vehicle-treated controls) for a range of genes of interest (X-axis) in pMBMECs. The treatment groups include cells exposed to 2 µM or 5 µM flecainide or PBS vehicle control 24 h prior to cell harvesting. The bars represent the mean ΔΔCt values while error bars indicate standard error of the mean (SEM) across three independent experiments. (B) Representative Western blot images showing protein expression of integrin β-3, JAM2, and JAM3 in pMBMECs treated with flecainide (2 µM or 5 µM) or vehicle. (C) Densitometric quantification of protein expression normalized to β-actin and additionally to the vehicle-treated control group. Statistical significance was determined using two-way ANOVA followed by Bonferroni´s post-hoc test. Asterisks denote significant differences compared to the vehicle-treated group at *p < 0.05 and ***p < 0.001.

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