Table 4 List of primers used for qPCR analysis.

From: Flecainide mediated sodium channel blockade enhances blood brain barrier integrity and promotes neuroprotection in neuroinflammation

V-CAM-1

JAM-1

  Forward sequence GCTATGAGGATGGAAGACTCTGG

Forward sequence CACCTACTCTGGCTTCTCCTCT

  Reverse sequence ACTTGTGCAGCCACCTGAGATC

Reverse SEQUENCE TGCCACTGGATGAGAAGGTGAC

I-CAM-1

JAM-2

  Forward sequence AAACCAGACCCTGGAACTGCAC

Forward sequence CAGACTGGAGTGGAAGAAGGTG

  Reverse sequence GCCTGGCATTTCAGAGTCTGCT

Reverse sequence GCTGACTTCACAGCGATACTCTC

I-CAM-2

JAM-3

  Forward sequence GACGGTCTCAACTTTTCCTGCC

Forward sequence GCATTGCTTCCAATGACGCAGG

  Reverse sequence CCATTTGGTTGTCCTGCATCGG

Reverse sequence GATGAAGCAGCCTCGTCTGTAC

PECAM-1

Occludin

  Forward sequence CCAAAGCCAGTAGCATCATGGTC

Forward sequence TGGCAAGCGATCATACCCAGAG

  Reverse sequence GGATGGTGAAGTTGGCTACAGG

Reverse sequence CTGCCTGAAGTCATCCACACTC

NaV1.5

NaV1.9

  Forward sequence GGTTCCGAGATGGTCAGTTGCT

Forward sequence GTCTTCAGCATGTTCATTATC

  Reverse sequence CAAGGACTCCTGTCCAATACGG

Reverse sequence CCACACATTATGAAGTCCTCG

NaV1.6

GAPDH

  Forward sequence AAGTGGACAGCCTATGGCTTC

Forward sequence TGTGTCCGTCGTGGATCTGA

  Reverse sequence AGCCAGAAGATGAGACACACC

Reverse sequence CCTGCTTCACCACCTTCTTGA

Back to article page