Fig. 5
The colony-forming potential of AP-expanded CD34+ cells. (A) Representative images of colony formation (BFU-E, CFU-GM and CFU-GEMM) in Methocult® media following 14 days of culture. (B) The colony-forming potential of expanded CD34+ cells after culture with cytokines alone or 1, 2.5 or 5 µM AP. Colonies were counted according to colony size and cellular composition. Abbreviations: BFU-E, erythroid burst forming units; CFU-GM, granulocyte/macrophage common precursors. Comparing each colony between group: * represents CFU-E colony, # represents BFU-E colony, $ represents CFU-GM colony and γ represents CFU-GEMM colony). Data is represented as mean ± SEM from three independent experiments. Data is represented as mean ± SEM from three independent experiments. *P < 0.05, #P < 0.05, $P < 0.05 and γP < 0.05, compared with control group for each day.
