Table 1 Human primer sequences used for quantitative RT-qPCR analysis.
Gene | Forward sequence (5′–3′) | Reverse sequence (5′–3′) |
|---|---|---|
CD34 | GGAAGGATGCTGGTCCG | CTGGGGTAGCAGTACCGTTG |
CD90 | CGCTCTCCTGCTAACAGTCTT | CAGGCTGAACTCGTACTGGA |
CD133 | CCATTGGCATTCTCTTTGAA | TTTGGATTCATATGCCTTCTGT |
CD117 | TGGGATTTTCTCTGCGTTCT | TGATTTTCCTGGATGGATGG |
ALDH1 | AATGGCATGATTCAGTGAGTGGC | GAGGAGTTTGCTCTGCTGGTTTG |
ALDH2 | CAAGATAGAGATGCCCGGCG | ACAGGGAACACTCTCCCACT |
BMI1 | CTTTCATTGTCTTTTCCGCC | TCCACAAAGCACACACATCA |
HOXB4 | CCTGGATGCGCAAAGTTCA | AATTCCTTCTCCAGCTCCAAGA |
GATA2 | CACAAGATGAATGGGCAGAA | ACAATTTGCACAACAGGTGC |
RUNX1 | CGATGGCTTCAGACAGCATA | GGCATCGTGGACGTCTCTA |
CXCR4 | CAGCAGGTAGCAAAGTGACG | CCCATTTCCTCGGTGTAGTT |
NOTCH1 | CCGCAGTTGTGCTCCTGAA | ACCTTGGCGGTCTCGTAGCT |
HES1 | CCAGCCAGTGTCAACACGA | AATGCCGGGAGCTATCTTTCT |
HES5 | TTCTCAGAGAATGTGTGTGCAGAGT | GGTCAGACACTTGGCAGAAGATG |
HEY1 | CTGCAGATGACCGTGGATCA | CAACTTCTGCCAGGCATTCC |
CTNNB1 | GCTTGTTCGTGCACATCAGGA | TGTGAACATCCCGAGCTAGGA |
WNT6 | TCTGGATACTGGGCTCCCC | ATTGATACTAACCTCACCCACC |
AXIN2 | CCTGGCTCCAGAAGATCACA | AGCATCCTCCGGTATGGAAT |
LEF1 | GCCAGACAAGCACAAACCTCT | TGGCATCATTATGTACCCGG |
FZD2 | GTCCTCAAGGTGCCATCCTATC | GCGTCCCTCCTGTGAGAAGAA |
CCDN1 | GATCAAGTGTGACCCGGACTG | CCTTGGGGTCCATGTTCTGC |
CD44 | CCAACTCTAATGTCAATCGTTC | GCCAAGATGATCAGCCATTCTG |
c-MYC | ATGGCCCATTACAAAGCCG | TTTCTGGAGTAGCAGCTCCTAA |
GAPDH | GAGTCAACGGATTTGGTCGT | TTGATTTTGGAGGGATCTCG |
