Fig. 6
Effect of Salvia fruticosa extract on U2OS osteosarcoma cell migration and wound closure over 12, 24, and 48 h of incubation. (a) Comparisons between treated and untreated cells revealed no significant difference in the wound closure between 12 and 24 h of incubation while the extract inhibited cell migration, with almost complete wound closure in untreated cells after 48 h. These results were supported by the statistical analysis in (b) where the extract’s IC50 concentration showed no significant decrease in wound closure percentage compared to the control (untreated cells) at the 12- and 24-hour time points. However, a significant decrease of nearly 43 ± 8.39% in wound closure was observed after 48 h. This suggests the extract’s potential to inhibit cell metastasis. Images were captured using an inverted microscope with a 20X magnification lens. Data represents three independent experiments, and significance analysis was performed using two-way ANOVA followed by Tukey’s multiple comparisons test at a 95% confidence level in GraphPad Prism 9 (**p < 0.01, ns = no significance).
