Fig. 2 | Scientific Reports

Fig. 2

From: Integration of biomimetic organoid-on-chip and 2D models advances the mechanistic Understanding of STEAP3-mediated regulation in intestinal viral infection

Fig. 2

STEAP3 knockdown enhances virus infection by modulating viral entry. (A) The STEAP3 mRNA level was measured in RD cells transfected with control or STEAP3-targeting siRNA. (B) The virus titer was measured from siRNA-transfected RD cells infected with EV-A71 (MOI = 1) for 48 h. (C) siControl or siSTEAP3-transfected RD cells were incubated with EV-A71 (MOI = 1) at 4 °C for 1 h. The total viral RNA was collected and measured using a real-time polymerase chain reaction (PCR). (D) siControl or siSTEAP3-transfected RD cells were infected with EV-A71 (MOI = 1) at 4 °C for 1 h and at 37 °C for another 1 h. The total viral RNA was collected and analyzed using real-time PCR. (E) Caco-2 cells were transfected with control or STEAP3-targeting siRNA and infected with SARS-CoV-2 S entry virus (1.1 × 10⁶ relative infection units) for 24 h. The total cell lysate was collected, and the luciferase activity was measured. Luciferase activity was quantified by the luciferase activity/protein concentration ratio (n = 3). (F) siControl or siSTEAP3-transfected calu-3 cells were infected with SARS-CoV-2 (MOI = 10−1) for the indicated times. The viral RNA was extracted and analyzed using real-time PCR (n = 3). *p < 0.05 versus siControl-transfected cells using the student’s t-test. All data are shown as the mean (± s.d.).

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