Fig. 3
From: The antitumor effect of tlr4 inhibition in head and neck cancer cell lines
Reduction in the proliferation and colony formation of HNC cell lines after TLR4 inhibition. (A) Proliferation assay. At 0 h, 1 × 104, 3 × 104, 1 × 104, or 4 × 104 FaDu, SCC78, SCC143, SCC154, or FaDu cells were seeded in a 24-well plate. After 24 h (arrow), the cells were treated with 10 µg/mL LPS or the corresponding IC50 concentration of TAK242 and counted at 48 h, 72 h, and 96 h. The results are from at least four independent replicates. (B) Colony formation assay. The cells were plated at low confluence and treated with LPS (10 µg/mL) or TAK242 (IC50) for 72 h. After 10–15 days, the total area of the colonies in the plate was measured, and the results are presented relative to those of the control. The results are from at least three independent replicates. (C) Representative images of cell well plates stained with crystal violet for the colony formation assay. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
