Fig. 7
From: The antitumor effect of tlr4 inhibition in head and neck cancer cell lines
Evaluation of the effect of TLR4 modulation on cisplatin sensitivity. The cells were treated for 72 h with different combinations of LPS (10 µg/mL), TAK242 (corresponding to the IC50 value), cisplatin (corresponding to the IC50 value), and DMSO (vehicle), and the viability of (A) SCC78, (B) SCC143, and (C) SCC154 cells was measured. Comparisons were made between each treatment group and the control group (*) or between the combined treatments and both individual treatments (#). At least four replicates of the experiments were performed independently. In (D–F) the TAK242 + cisplatin combination was analyzed by Calcusyn software (RRID: SCR_020251) (Calcusyn v2.1, Biosoft Bioinformatics Software Platform) to determine the combination index (CI) in SCC78, SCC143 and SCC154, respectively. In the left graph, the Dose-effect curves of TAK242 (red curve), Cisplatin (green curve), and TAK242 + cisplatin (blue circles). In the right graphs, the combination index plot of TAK242 + Cisplatin indicating the CI value. ****, p < 0.0001; ##, p < 0.01.
