Fig. 3

DDOX partially rescued total α-Syn and phospho-α-Syn¹²⁹ phenotypes induced by α-Syn PFF. (A) Confocal microscopy images, with accompanying insets, of SH-α-Syn-RFP cells immunostained with a total-α-Syn mAb after 24 h treatment with α-Syn PFF, α-Syn PFF + DDOX, or DDOX alone. n = 6. (B) Quantification of the fluorescence intensity of total-α-Syn mAb immunosignal from microscopy images. (C) Confocal microscopy images of phospho-α-Syn¹²⁹ immunostainings, uncovered with a polyclonal antibody (pAb), of SH-α-Syn-RFP cells treated with α-Syn PFF in the presence or absence of DDOX (50 µM) for 24 h. n = 6. (D) Quantification of the intensity of phospho-α-Syn¹²⁹ antibody signal in each condition; *** and ** indicate significant differences with p < 0.001 and p < 0.01 vs. Control, respectively; ns denotes not significant. Scale bar = 5 μm. For (B) and (D), values are the mean of 3 independent experiments, and error bars represent ± SD.