Fig. 12 | Scientific Reports

Fig. 12

From: A 2D inflammatory co-culture model for investigating synovial fibroblast and macrophage interactions in rheumatoid arthritis

Fig. 12

Co-culture of synovial FLS and macrophage lineage cells; (A) CLSM images representing ROS production in FLS co-cultured with either M0 or M1 macrophages at varying ratios (FLS: macrophage = 2:1, 1:1 or 1:2) on day 3; ROS is visualized in green (DCFDA), and nuclei are counterstained with DAPI (blue). Scale bar = 20 μm. (B) Quantitative analysis of ROS fluorescence intensity from CLSM images (n = 3; Two-way ANOVA, *p < 0.05).

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