Fig. 6
Gene and protein expression profiles of non–stimulated and cytokine-stimulated rFLS cells. (A, B) Gene expression of synovial fibroblast markers Thy–1 and cadherin–11; (C–E) Gene expression of proinflammatory cytokines TNF–α, IL–1β and IL–6; (F–H) ELISA-based quantification of TNF–α, IL–1β and IL–6 secretion in non-stimulated vs. stimulated rFLS cells and (I) Cytokine concentrations in the macrophage-derived conditioned media used for FLS stimulation; (J–L) Gene expression of CD44, MMP2 and MMP9, in non-stimulated vs. stimulated rFLS cells (n = 3; Student’s t–test, *p < 0.05).
