Fig. 1 | Scientific Reports

Fig. 1

From: MIEN1 promoter deletion leads to impaired migration and invasion potential via actin cytoskeleton rearrangement in colorectal cancer

Fig. 1

MIEN1 promoter knockout and cell line characterization. (A) Schematic representation of chromosome 17 of the human genome, depicting the location of the MIEN1 gene. (B) Genomic DNA sequencing of HT29 cells following the deletion of the MIEN1 promoter region. (C) Reverse transcriptase polymerase chain reaction of MIEN1 in both HT29 cell variants, WT and KO, observed at 200 bp, and the GAPDH gene observed at 300 bp as a control. (D) Western blot image representative of the whole-cell lysate of HT29 WT and KO cells showing the expression of MIEN1, ErbB2, and GRB7 proteins, which represent the protein products of genes adjacent to MIEN1. The Hsc70 protein was used as a loading control. (E) Quantification of the band density of western blot images for proteins to compare HT29 WT and KO cell expression. The paired t-test was used to perform statistical analysis, with n = 5 in each group. ns, non-significant; ***, p < 0.0005; MIEN1, migration and invasion enhancer 1; bp, base pairs; WT, wild-type; KO, knockout.

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