Fig. 2

Effect of LDR and malathion single and co-exposure on neuronal survival, inflammation, and maturation. (a) Representative Nissl-stained mouse hippocampus; (b) Number of pyknotic neurons in different groups . (c) Representative image showing NeuN staining for mature neurons; (d) Percentage of NeuN-positive area in the DG region; (e) Percentage of NeuN-positive area in the CA3 region. (f) Representative image showing GFAP staining for astroglia; (g) Number of reactive astroglia in the DG region; (h) Graph representing the number of reactive astroglia in the CA region. (i) Representative image showing Iba-1 staining for microglia; (j) Number of resting microglia in the hippocampus; (k) Number of activated microglia in the hippocampus (n = 3, p< *0.05, **0.01, ***0.001, data represented as Mean ± SEM) (black arrow indicate positively stained cells, images in 40X).