Table 1 The primers and probe used for the identification of Fangshan bunya-like virus in this study.
From: Genome of a novel Fangshan bunya-like virus identified in mosquitoes from Shandong Province, China
Virus | Type | Primer/probe | Sequence (5’ to 3’) |
|---|---|---|---|
Fangshan bunya-like virus | qRT-PCR | F | GAGTGTTACAAAGCTCTGATGCCAT |
R | TGTGCAGTTCAACATGATCCATG | ||
P | FAM-CCATAGAAGCCACCCTTGAGAAATCACCG-bhq1 | ||
Nested PCR | Outer-F | GGTGAGAGTTGATGAGTCAGAGGTT | |
Outer-R | TTACTCACCAGCCTGCTCCATT | ||
Inner-F | GTATCGCAAGAGGAATCAGGAGTC | ||
Inner-R | CCTCCAGAAGCTCATCGTCAAT |
