Fig. 1
From: Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM
MPhoto analyses of GPBAR1 alone and the GPBAR1/Gs complex. (A) Schematic representation of the GPBAR1/Gs complex. The different G protein subunits are shown: Gαs in light green (43 kDa), Gβ in dark green (39 kDa), and Gγ (8 kDa) in yellow. The complex is stabilized by Nb35 (17 kDa), shown in cyan. (B). Histogram of the mass distribution of the GPBAR1 for a final concentration at 10 nM. (C) Histogram of the mass distribution of the GPBAR1/Gs complex for a final concentration at 30 nM. The continuous curve corresponds to the mass distributions of the majority of species fitted with a Gaussian function.
