Fig. 2
From: Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM
Cryo-EM map and atomic model. (a) Cryo-EM map at 2.5 Å of the GPBAR1 G protein complex, the map has been colored according to the different sub-units. (b) Cryo-EM map at 2.9 Å with the flexible AH domain. (c, d) Atomic model of the two complexes placed in the density. (d) The flexible Gαs part is surrounded by dotted lines. (e) Important regions for the interaction between GPBAR1 and Gαs. (f) P395 and the interacting residues of the ligand pocket, the backbones is rainbow colored (Blue N-ter Red C-ter). (g) Contact residues between GPBAR1 and the Gαs α5 helix. (h) Contact residues between TM5 and ICL3 of GPBAR1 with α4 and i3L of Gαs.
