Fig. 2

Apoptosis/necrosis detection by image cytometry. MCF-7 cells were treated with 1 µM DOX or 80 µM DT and combined, after 48 h incubation. Control cultures were treated with DMSO (0.5%) as a vehicle a. Representative histograms of three independently performed. Q1II—live, Q1Ir—early apoptotic, Q1ur—late apoptotic, and Q1uI—necrotic cells. b. The data derived from three independent experiments reported as the mean ± SD. DOX: doxorubicin; DT: diosmetin; DT + DOX: diosmetin + doxorubicin. p < 0.05 vs. control, ^p < 0.05 vs. DOX alone.