Fig. 1

Phylogenetic reconstruction based on the complete polyprotein (amino acid sequences) of the Orthoflavivirus genus. The tree is midpoint-rooted, and the values near the nodes represent the bootstrap support values. Orthoflavivirus zikaense sequences are highlighted in brown. The analyses were conducted using twelve viral sequences: ZIKV_KU365777, ZIKV_DQ859059, and ZIKV_KU527068 (Orthoflavivirus zikaense); SOPV_DQ859064 (Spondweni virus); KEDV_DQ859061 (Orthoflavivirus kedougouense); DENV4_AY618991 (Orthoflavivirus denguei serotype 4); DENV2_JX079694 and DENV2_EF105385 (Orthoflavivirus denguei serotype 2); DENV3_AY648961 and DENV3_EU482458 (Orthoflavivirus denguei serotype 3); and DENV1_GQ199877 and DENV1_EU482822 (Orthoflavivirus denguei serotype 1).