Fig. 6
Normalized IgG reactivity profiles against the ZIKV polyprotein and correlation of epitope recognition between mother–newborn pairs. (A) and (B) Normalized spot intensities (y-axis) for each peptide (x-axis, spot number) across the ZIKV polyprotein are shown for Mother 87 and Newborn 87 (left), and Mother 128 and Newborn 128 (right). The red dashed line indicates the reactivity threshold (0.7). Peptides above this cut-off were considered potentially reactive. The schematic below each graph maps spot numbers to ZIKV protein domains. Bottom panels show Pearson correlation plots of normalized spot intensities between mothers and their respective newborns. Each dot represents one peptide. Strong positive correlations were observed for both dyads: r = 0.89 for Mother–Newborn 87 and r = 0.90 for Mother–Newborn 128 (both p < 0.0001), indicating substantial overlap in epitope recognition patterns, consistent with vertical antibody transfer.
